Liquid-chromatographic determination of progesterone in serum, with spectrophotometric detection.

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Liquid-chromatographic determination of progesterone in serum, with spectrophotometric detection.

In this precise, accurate, and specific liquid-chromatographic procedure for determining progesterone in serum, the serum is diluted 10-fold with water/methanol (65/35 by vol), and the progesterone is extracted from the sample by passage through a column of graphitized carbon black (Carbopack B, Supelco). After washing the column, we elute the progesterone with chloroform/methanol (90/10 by vol...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1986

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/32.3.508